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細胞名稱:人黑色素瘤細胞系M14
產品規格:T25培養瓶x1;1.5ml凍存管x2
細胞數量:1x10^6;1x10^6
保存溫度:37℃;-198℃
運輸方式:常溫保溫運輸;干冰運輸
安全等級:1
用途限制:僅供科研2類
培養體系:90%DMEM+10%FBS+1%三抗
培養溫度:37℃
二氧化碳濃度:5%
簡介:人黑色素瘤細胞系M14取自33歲男性供體。貼壁培養。
注釋:Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:MD Anderson Cell Lines Project.
Part of:NCI-60 cancer cell line panel.
Doubling time:26.3 hours(NCI-DTP).
Microsatellite instability:Stable(MSS)(Sanger).
Omics:Array-based CGH.
Omics:CNV analysis.
Omics:Deep exome analysis.
Omics:Deep proteome analysis.
Omics:DNA methylation analysis.
Omics:Fluorescence phenotype profiling.
Omics:lncRNA expression profiling.
Omics:Metabolome analysis.
Omics:Protein expression by reverse-phase protein arrays.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
基因突變
Heterozygous for BRAF p.Val600Glu(c.1799T>A)(ClinVar=VCV000013961)(PubMed=12068308;PubMed=17088437;PubMed=19799798;PubMed=29492214).
Heterozygous for CDKN2A c.150+2T>C(IVS1+2T>C);splice donor mutation and c.455insCdel26(PubMed=19799798;PubMed=29492214).
Heterozygous for TP53 p.Gly266Glu(c.797G>A)(ClinVar=VCV000161516)(PubMed=17088437;PubMed=18277095).
HLA信息
Class I
HLA-A A*11:01:01,24:02:01
HLA-B B*15,35
HLA-C C*03:03:01,04
Class II
HLA-DP DPB1*13:01,19:01
HLA-DQ DQB1*03:02,06:03
HLA-DR DRB1*04:05:01,13:01:01
STR信息
Amelogenin X
CSF1PO 11
D1S1656 11,12
D2S441 11,14